VITAMIN D level cassette test (whole blood from fingertip).

This is a rapid chromatographic immunological test used for the semi-quantitative detection of 25-hydroxyvitamin D (25 (OH) D) in whole blood from a fingertip. The test provides a preliminary result for diagnostic purposes and can be used for screening for vitamin D deficiency.

Vitamin D is a group of fat-soluble secosteroids responsible for increasing the absorption of calcium, iron, magnesium, phosphates, and zinc by the intestines. The most important compounds in this group for humans are vitamin D3 and vitamin D2. Vitamin D3 is naturally produced in human skin when exposed to ultraviolet radiation, while vitamin D2 is mainly obtained from food.

Vitamin deficiency can lead to various serious diseases, including osteoporosis, osteomalacia, multiple sclerosis, cardiovascular diseases, pregnancy complications, diabetes, strokes, autoimmune diseases, influenza, various cancers, infectious diseases, Alzheimer’s disease, obesity, and higher mortality.

OPERATING PRINCIPLE

The vitamin D level test is an immunological test based on the principle of competitive binding. During the test, the mixture migrates up the chromatographic membrane due to capillary action. The membrane is pre-coated with 25 (OH) D antigens in the test line area. During the test, 25 (OH) D present in the sample will compete with 25 (OH) D on the test line for a limited amount of anti-25 OH vitamin D antibodies in conjugation. The higher the concentration of 25 (OH) D in the sample, the clearer the T line will be. The result is read according to the color chart included in the kit. As part of procedural control, the red line will always appear in the control line area (C), indicating whether the correct volume of the sample has been added and whether the membrane has been moistened.

More information in the leaflet – available upon request.

Product intended for self-monitoring.